Cbmmunication Inactiyation of Glutamate Racemase of Pedioeoecus pentosaceus with L - Serine O - Sulfate
نویسندگان
چکیده
peptidoglycan layer of the bacterial cell wall,3) and accordingly can be a target fbr development of new antibiotics. We purified the enzyme from Pediococcus pentosaeeus4) and cloned its gene into Eycherichia colii'2} to construct an overproducer,S) We here report that L-serine O-sulfate, an analog of L-glutamate, serves as a suicide substrate to inactivate the enzyme, This is thc first example
منابع مشابه
The Escherichia coli Dga (MurI) protein shares biological activity and structural domains with the Pediococcus pentosaceus glutamate racemase.
The Pediococcus pentosaceus glutamate racemase gene product complemented the D-glutamate auxotrophy of Escherichia coli WM335. Amino acid sequence analysis of the two proteins revealed 28% identity, primarily in six clusters scattered throughout the sequence. Further analyses indicated secondary structure similarities between the two proteins. These data support a recent report that the dga (mu...
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